Selectin E/CD62e antibody

Catalog No. ABIN112379

The Rat Monoclonal anti-Selectin E/CD62e antibody has been validated for WB, FACS, IF and Func. It is suitable to detect Selectin E/CD62e in samples from Human and Mouse.

Quick Overview for Selectin E/CD62e antibody (ABIN112379)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: UZ4

Product Details anti-Selectin E/CD62e Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Purified

Immunogen: LPS-activated mlEND1 cells expressing E-selectin at their cell surface

Isotype: IgM

Application Details

Application Notes: UZ4 is a functional antibody in that it inhibits leukocyte adhesion to both murine andhuman activated endothelial cells. For in vivo application in mice 20 g of purified Ig perinjection is recommended. UZ4 is also suitable for immunoblotting, ELISA, immunoprecipitation and flow cytometry(dilution 1: 500). It is not optimal for immunohistochemistry, to analyze mouse E-selectin protein in frozensections, please refer to the anti E-selectin monoclonal antibodies UZ5 (ABIN112380), UZ6(ABIN112381), and UZ7 (ABIN112382).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: CD62E / E-Selectin

Background: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF-alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

Gene ID: 9606

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling